Detection and Quantitation of Wilms’ Tumor 1 Gene Transcripts

Measurable Residual Disease (MRD) is an important factor for risk stratification and treatment planning for AML patients. MRD could be evaluated according to different parameters including AML related gene expression. According to current LeukemiaNet guidelines, when no other parameter is available, Wilms’ Tumor 1 Gene (WT1) expression may be used as MRD marker (A Hochhaus, 2020, Leukemia, 34:966).
WT1 RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of WT1 and ABL transcripts.

Category: Oncology biomarkers

Advantages of WT1 RQ Kit

Ready to Use

The WT1 and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for WT1 and Yellow/VIC channel for ABL.

Control Gene

Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis, preventing related false negative results.

Quality Control

Standards & mixes of WT1 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 2 copies/μl or 0.04% for WT1 in the context of 10,000 copies/μl of ABL transcripts

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex (FAM, VIC)

Kit Contents:

Kit contains WT1 Mix and 5 quantitation standards for WT1, ABL Mix and 4 quantitation standards for ABL, PCR grade water, manual and a CD with template file.

Packaging:

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support:

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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