At NovinGene, our customers are our top priority. We are dedicated to understanding and meeting their unique needs with tailored solutions. Our team is committed to providing exceptional support and guidance every step of the way, ensuring our customers receive prompt assistance and expert advice. We actively listen to their feedback to continually improve our qPCR kits and enhance their performance. We view our customers as partners in advancing healthcare diagnostics; by fostering collaborative partnerships, we work together to tackle evolving challenges in diagnostic testing and improve patient outcomes worldwide.
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No, all kit components should be stored at -10 to -30°C. Defreeze and use reagents while kept on crushed ice.
If Kit contents/reagents are warmed up to 10°C or higher, results would be unreliable.
No, keeping kits in fridge even for a short time is not recommended. In such case, results would be unreliable.
Kits should be shipped and stored at -10 to -30°C. This will ensure kits stability till the expiry date.
If the cooling block is equipped with thermometer, kit reagents can be stored on it while it maintains a temperature below 0°C. Please note that, block without thermometer are not suitable as they get warmed without noticing.
In this case, the kit sensitivity and specificity will be reduced sharply and results will not be reliable and may include false-negative or false-positive.
It depends on number of controls/standards provided in the kit and number of samples to be examined as well as the platform. For example, HCV kit comes with 4 standards. If kit is to be used once, it would be enough for 19 patients along with 4 standards and one NTC with standard volume. If the kit is to be used twice, then only 14 samples could be examined. On some platforms like RotorGene, standard curve could be imported, so higher number of samples could be examined.
If the kit has just been opened, it is recommended to use all the standards as described in the manual. However, an experienced technician may use lower number of standards at his/her own discretion. After the first use, lower number of standards i.e. 1 to 3 could be used depending on the platform.
Using Rotorgene standard curve from a previous test could be used in the current test. To do so you should include at least one standard in the current test. Then for analyzing result select “improt curve” option from the previous run. For more information refer to RotorGene manual please.
Negative control contains DNA which is used to evaluate cross reaction and specificity. While the water/NTC is used to evaluate contamination.
If NTC is positive with CT of less than 35 it shows contamination with PCR product. Always avoid opening PCR tubes and discard them into 10% bleach container.
Technical support team is available in working hours by
email: novingene.tech.supp@gmail.com or by Phone/WhatsApp: +98-993 622 3241.
Test done on RotorGene, StepOne, MIC and LC96 can be examined by technical support team. To do so Test/Run/Experiment file should be sent by email or through whatsApp.
Email: novingene.tech.supp@gmail.com
WhatsApp: 09936223241
Quantitation standards are defined as copy/ul. To convert the results to copy/ml, use the following equation.
“Sample volume” is the plasma, blood, CSF, etc. volume used for DNA isolation and “Elution volume” is the volume of buffer or water used to elute or dissolve isolated DNA. For example, if 200ul or 0.2ml of sample is extracted and elution volume is 50ul, the result must be multiplied by 250 to be converted to copy/ml.
Currently, International units have been defined for HBV, HCV and HIV.
Using NovinGene kit, HBV IU equals to 7 copies, HCV IU equals to 4 copies and HIV IU equals to 1 copy.
In such case convert the standards titers to copy/ml as above described (question number 12).
Use the internal control (if provided) to examine the extraction quality. Some kits also do not need IC as they use human DNA as both target and IC.
If results are positive in Green/FAM channel, then results are good to be reported. However, if results are negative in Green/FAM channel, then results are inconclusive and the test should be repeated.
All patient samples should be positive in VIC/ABL channel with a sigmoid graph and CT of 27 or less. ABL CT greater than 27 usually happens if not enough cells have been extracted or less than 100ug RNA has been used. CTs higher than 27 reduce the sensitivity of the test and may result in false negative reports. It is recommended to use buffy-coat and to using RNA isolation with recommended kit below:
- TriPure isolation Reagent (Cat. no. 1667157, Roche Applied Science, and Mannheim, Germany).
- TRIzol isolation reagent (Cat. no. 15596026, Invitrogene/Thermo Fisher, USA)
- Isol-RNA isolation reagent (Cat. no. 2302700, 5 prime/ Thermo Fisher, Germany)
- Accuzol isolation reagent (Cat. no. K-3090, Bioneer, Korea)