Detection and Quantitation of Varicella Zoster Virus (VZV)

Varicella Zoster Virus (VZV) is a double-stranded 125 kb DNA virus and a member of Herpesviridae family. Varicella (chickenpox) is the result of childhood infection. Following the initial infection virus remains latent in nervous ganglia. Reactivation of the virus in adults results in Zoster (shingles). Infection during pregnancy may also lead to congenital defects.
VZV RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of Varicella Zoster virus.

Category: Infectious Diseases

Advantages of VZV RQ Kit

Ready to Use

The VZV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes
followed by the sample DNA. Results can be viewed in Green/FAM channel.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure, PCR inhibition or setup errors, preventing false negative results. Internal Control is detected in Yellow/VIC channel.

Quality Control

VZV Kits are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 4 copies/µl for the cloned target

Linear Range: 100 million copies/µl to 10 copies/µl for the cloned target

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains VZV Mix, Internal Control, 4 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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