Detection of Ureaplasma
Ureaplasma parvum and Ureaplasma urealyticum are classified under Mycoplasmataceae.These cell wall–deficient bacteria are commonly found in the urogenital tract of many individuals. Although they are often asymptomatic, in some cases they are associated with complications such as infertility, genital tract inflammation, and pregnancy-related conditions including preterm birth and chorioamnionitis.
Since, culturing Ureaplasma species is both difficult and time-consuming, molecular diagnostic methods particularly PCR and Real-Time PCR are recognized as the most reliable approaches for the detection. These techniques provide rapid, sensitive, and accurate identification, ensuring precise differentiation.
The Ureaplasma RG kit offers a ready-to-use Multiplex Real-Time PCR assay for the detection of Ureaplasma parvum and Ureaplasma urealyticum.
Category: Infectious Diseases
Description
Advantages of Ureaplasma RG Kit
Ready to Use
The Ureaplasma Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results are viewed in Green/FAM, Orange/ROX and Yellow/VIC channels.
Internal Control
This Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.
Quality Control
The Control and Mix components of the Ureaplasma RG Kit are manufactured in batches, with each batch being distributed only after successfully passing Quality Control tests for specificity, sensitivity, accuracy, and precision.
Easy & Quick Setup
Kit contains a template file for quick and easy setup of the Real-Time PCR instrument, also reducing setup errors.
Kit specifications
Analytical sensitivity: 7.7 Copies/µl for Ureaplasma parvum and 7.5 Copies/µl for Ureaplasma urealyticum
Reaction type: Qualitative Real-Time PCR
Detection Method: TaqMan probe, Multiplex (FAM, Orange and VIC)
Kit Contents
Kit contains PCR Mix and positive control and PCR grade water, manual, quick guide and a CD with template file.
Packaging
Kit is available as 24, 48 and 96 reactions of 25µl.
Technical Support
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.
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