Quantitative Detection of M. Tuberculosis 

Tuberculosis (TB) is one of the major public health problems worldwide and results in about 2 million deaths annually. This infection is caused by a group of closely related species and subspecies of intracellular pathogens called Mycobacterium tuberculosis complex (MTC) including M. tuberculosis, M. bovis and M. bovis BCG.

While bacterial culturing is still the gold standard for TB diagnosis, Real-Time PCR is increasingly used as an alternative due to its rapid, sensitive and specific results. It also provides the highest sensitivity and widest dynamic range among other methods. 

TB RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of Mycobacterium tuberculosis complex DNA.

Category: Infectious Diseases

Advantages of TB RQ Kit

Ready to Use

The TB Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel and yellow/VIC channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

TB standards & mix are manufactured in batches, and each series is distributed after passing quality control tests for specificity, sensitivity, accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 1 copy/µl for the cloned target

Linear Range: 1 million to 10 copies/µl for the cloned target

Reaction type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains TB Mix, Internal Control, 5 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or trouble shooting.

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