Detection and Quantitation of PML-RARA (bcr1) Transcripts

PML-RARA is an abnormality resulted from t(15;17) (q22; q21) translocation. This translocation results in fusion of PML (promyelocytic) gene with RARA (retinoic acid receptor alpha) gene, and production of chimeric PML-RARA protein which is a transcription repressor and impairs the myeloid differentiation. While RARA breakpoints always occur in intron 2, PML breakpoints involves three different regions of intron 6 (55%), exon 6 (5%) and intron 3 (40%). The resulted isoforms of PML-RARA are respectively called bcr1/Long/L, bcr2/Variant/V and bcr3/Short/S.
PML-RARA accounts for more than 90% of APL (acute progranulocytic leukemia) cases, 10%-15% of AML (acute myeloid leukemia) cases.
PML-RARA (bcr1) RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of PML-RARA (bcr1) transcripts and monitoring MRD.

Category: Oncology biomarkers

Advantages of PML-RARA (bcr1) RQ Kit

Ready to Use

The bcr1 and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for bcr1 and Yellow/VIC channel for ABL.

Control Gene

Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis, preventing related false negative results.

Quality Control

Standards & mixes of bcr1 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 2 copies/µl or 0.02% for bcr1 in the context of 10,000 copies/µl of ABL transcripts. 

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains bcr1 Mix and 5 quantitation standards for bcr1, ABL Mix and 4 quantitation standards for ABL, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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