For Detection of PML-RARA (bcr1, bcr2, bcr3) Transcripts

PML-RARA is an abnormality resulted from t(15;17) (q22; q21) translocation. This translocation results in fusion of PML (promyelocytic) gene with RARA (retinoic acid receptor alpha) gene, and production of chimeric PML-RARA protein which is a transcription repressor and impairs the myeloid differentiation. While RARA breakpoints always occur in intron 2, PML breakpoints involves three different regions of intron 6 (55%), exon 6 (5%) and intron 3 (40%). The resulted isoforms of PML-RARA are respectively called bcr1/Long/L, bcr2/Variant/V and bcr3/Short/S.
PML-RARA accounts for more than 90% of APL (acute progranulocytic leukemia) cases, 10%-15% of AML (acute myeloid leukemia) cases.
PML-RARA 3X RQ kit provides a ready-to-use Real-Time PCR assay for detection of bcr1, bcr2 and bcr3 transcripts.

Category: Oncology biomarkers

Advantages of PML-RARA 3X RQ Kit

Ready to Use

All Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for bcr1,bcr2, bcr3 and Yellow/VIC channel for ABL.

Control Gene

Kit also provides ABL Mix and positive control for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis, preventing related false negative results.

Quality Control

Mixes and Control of PML-RARA 3X RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 2 copies/µl or 0.02% for bcr in the context of 10,000 copies/µl of ABL transcripts. 

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains bcr1 Mix, bcr2 Mix, bcr3 Mix, ABL Mix and one Positive Control, PCR grade water, manual and a flash card with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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