Parvovirus B19 is a member of Parvoviridae family with a non-enveloped icosahedral capsid and single-stranded DNA genome. This virus causes Erythema infectiosum or Fifth Disease, which is more common in children and usually has mild symptoms like mild rash on the cheeks. The main transmission route is through respiratory droplets while, it is also transmitted to the fetus during pregnancy. Since fetal infection can lead to serious consequences including fetal loss, early diagnosis during pregnancy is important.
 Parvovirus B19 RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of  Parvovirus B19.

Category: Infectious Diseases

Advantages of Parvovirus B19 RQ Kit

Ready to Use

The PV-B19 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control
PV-B19 standards & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 4 copy/µl for the cloned target

Linear Range: 10 million copy/μl to 10 copy/μl

Reaction type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PV-B19 Mix, Internal Control, 5 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

Documents: