Detection of PAI-1 4G/5G polymorphism

PAI-1 (Plasminogen Activator Inhibitor 1) is a 52 kDa glycoprotein. It is the main inhibitor of activators of plasminogen, and hence fibrinolysis and blood clot degradation. PAI-1 gene is located on chromosome 7 and consists of 9 exons. It includes many variations. The most significant variation, however, lies in the promoter region at -675, which is a single guanine base insertion/deletion known as 4G/5G polymorphism. While 5G allele binds both transcription activator and repressor, 4G allele only binds activator and leads to higher expression of PAI-1. 4G allele is considered a predisposing factor and has been related to various clinically important conditions including deep vein thrombosis, atherosclerosis and myocardial infarction, cancer, sepsis, miscarriage and asthma.

PAI-1 RQ kit provides a ready-to-use Real-Time PCR assay for detection of PAI-1 4G/5G polymorphism.

Category: Genetic Factors

Advantages of  PAI-1 RQ Kit

Ready to Use

The PAI-1 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be simply viewed by scatter graph analysis or other methods.

Internal Control

Due to dual function of the PCR Mix, the main reaction ensures quality of patient sample and DNA extraction and prevents false negative results by PCR inhibition or setup errors.

Quality Control

Mix & controls of PAI-1 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Differentiation: 4G/4G, 4G/5G and 5G/5G Genotype

Reaction Type: Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PAI-1 Mix, 4G/4G Positive control, 4G/5G Positive control, 5G/5G Negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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