Detection of NRAS Mutations

The NRAS oncogene (Neuroblastoma RAS viral oncogene homolog) belongs to the RAS family and plays an important role in regulating cell division and cellular differentiation as a protein kinase. Mutations in this oncogene contribute to the development of various cancers, including melanoma, acute myeloid leukemia, colorectal cancer, and thyroid cancer. According to reports, NRAS mutations are observed in more than 10% of patients with Acute Myeloid Leukemia (AML). This oncogene is located on chromosome 1,  exon 2 (codons 12 and 13), exon 3 (codons 59–61), exon 4 (codons 117 and 146).

Category: Oncology biomarkers

Advantages of NRAS RQ Kit

Ready to Use

The NRAS Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Quality Control

NRAS RQ Kit is manufactured in batches, and each series is distributed only after careful Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument.

Kit Specifications

Analytical Sensitivity: 0.5% – 1.2%, Depending on the mutation type

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex/ Duplex (FAM, VIC)

Kit Contents

Kit contains two Control Mixes and seven PCR Mixes including G12, G13, A59D, Q61H, Q61K, Q61L, Q61R for NRAS mutations, positive control, negative control, PCR grade water, manual and template file.

Packaging

Kit is available as 24 and 48 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

 Documents: