Detection of KRAS Mutations

Colorectal Cancer (CRC) is among the most prevalent cancers worldwide. Treatment with monoclonal antibodies against Epidermal Growth Factor Receptor (Anti-EGFR) has shown to be effective for CRC patients. However, Anti-EGFR would be ineffective in presence of some KRAS mutations including mutations in codons 12 and 13.
This is the same for treating Non-Small Cell Lung Cancer (NSCLC) with Anti-EGFR. Therefore, determining the KRAS mutation status is essential for these patients. It should be noted that, 95% and 88% of KRAS mutations in CRC and NSCLC patients respectively are reported in codons 12 and 13 including G12A, G12C, G12D, G12R, G12S, G12V and G13D.
KRAS RQ kit provides a ready-to-use Real-Time PCR assay for detection of these 7 mutations.

Category: Oncology biomarkers

Advantages of KRAS RQ Kit

Ready to Use

The KRAS Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Quality Control

Mixes of KRAS RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 1%-4%, Depending on the mutation.

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex/Duplex (FAM, VIC)

Kit Contents

Kit contains 7 PCR Mixes (G12A, G12C, G12D, G12R, G12S, G12V, G13D) and positive control, negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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