Detection of JAK2 Mutation (V617F)

JAK2 (Janus Kinase 2) is a Tyrosine Kinase located in cytoplasm with essential role in signaling pathways for cytokines and growth factors. The acquired mutation G1849T replaces valine with phenylalanine (V617F). This substitution results in constitutively active JAK2 which leads to uncontrolled cell proliferation in the absence of growth factors. This mutation is found in the majority of BCR-ABL-negative myeloproliferative disorders (MPDs) and has become a main diagnostic test for polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF).

JAK2 RQ kit provides a ready-to-use Real-Time PCR assay for detection of JAK2 V617F mutation.

Category: Oncology biomarkers

Advantages of JAK2 RQ Kit

Ready to Use

The JAK2 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel.

Internal Control

The PCR mix detects also a housekeeping gene in Yellow/VIC channel. Internal Control ensures quality of patient sample and DNA extraction and also prevents false negative results by PCR inhibition or setup errors.

Quality Control

JAK2 controls & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 0.1 % in 10-50ng/ul DNA

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains JAK2 Mix, Positive control, Negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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