Detection and Quantitation of HTLV-I

Human T-cell Lymphotropic Virus I (HTLV-I) is the first human retrovirus discovered (1979). Two years later HTLV-II was also discovered. About 15 to 20 million people are infected with these viruses worldwide. Transmission is mostly through sexual contact, breastfeeding, transfusion, transplant and intravenous drug use.
Within the body, infection spreads slowly through virological synapses from cell to cell and takes 20-50 years to develop symptoms. Infection with HTLV-I may lead to leukemia/lymphoma or myelopathy while HTLV-II causes mild neurologic disorders or chronic pulmonary infection. Disease progress and pathogenesis depend partly on proviral load and monitoring that could be beneficial for patient management.
HTLV-I RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of HTLV-I provirus and cellular titer based on Albumin gene. The Albumin gene also monitors extraction quality and prevents false negative results.

Category: Infectious Diseases

Advantages of HTLV RQ Kit

Ready to Use

Both of the HTLV-I Mix and Albumin Mix contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results for HTLV-I can be viewed in Green/FAM channel.

Internal Control

Kit also contains a second set of PCR mix and standards for Albumin as External Control and normalizer. Results can be viewed in Yellow/VIC channel and provides sample cellular load to normalize HTLV-I titer. This Control also prevents false negative results due to extraction failure, PCR inhibition or setup errors.

Quality Control

HTLV-Albumin standards & mixes are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR machine and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 2 copies/µl for HTLV-I and 1 copy/µl for Albumin

Linear Range: 1 million to 10 copies/µl for the cloned target

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains HTLV-I Mix and Albumin Mix, five standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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