Detection and Differentiating of HSV-1 and HSV-2

Human Herpes Virus type 1 and 2 (HHV-1, HHV-2) known as Herpes Simplex Virus 1 and 2 (HSV-1, HSV-2) are large, enveloped DNA viruses with a genome of about 150 kbp double stranded DNA. HSV is a ubiquitous agent and can cause a variety of infections. Although most HSV infections in adults are usually benign, yet HSV is the most common cause of viral encephalitis and genital herpes is one of the most prevalent sexually transmitted diseases (STDs). Despite overlapping clinical manifestations, HSV-1 and HSV-2 infections are different regarding the severity and outcome, mortality, frequency of reactivation, response to medication, and drug resistance. Therefore, defining the type is clinically important and routinely recommended.
Classically HSV-1 and HSV-2 infections are associated with oral or genital diseases, respectively. However, the differentiation of HSV-1 from HSV-2 based on anatomical site of infection is far from absolute and both HSV-1 and -2 can be isolated from skin, mucous, and visceral organs. Also, HSV-1 has become the predominant cause of
genital ulcers in some developed countries. Therefore, HSV1 and HSV-2 detection and typing based on clinical symptoms is not reliable
and requires laboratory tests. Also, in case of viral encephalitis, prompt laboratory diagnosis of HSV infection is essential for patient
management and possible initiation of antiviral therapy and reducing the mortality rate. HSV Typ RQ kit serves the both of above, fast and highly sensitive detection and typing of HSV-1 and HSV-2 infections based on Real-Time PCR machines.

Category: Infectious Diseases

Advantages of HSV Type RQ Kit

Ready to Use

The HSV Type Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM, Yellow/VIC and Orange/ROX channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

HSV Type Control & mix are manufactured in batches, and each series is distributed after passing quality control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 0.5 copy/µl for the cloned target

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Triplex (FAM, VIC, ROX)

Kit Contents

Kit contains HSV Typ Mix, Internal Control, one Positive control for each of types, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25 µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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