Detection and Quantitation of Herpes Simplex Virus 1 and 2

Human Herpes Virus type 1 and 2 (HHV-1, HHV-2) known as Herpes simplex virus 1 and 2 (HSV-1, HSV-2) are large, enveloped DNA viruses with a genome of about 150 kbp double stranded DNA. HSV is a ubiquitous agent and can cause a variety of infections which in adults are usually benign. Following active infection, it establishes a latent infection in the sensory and autonomic ganglia of the nervous system. It can be reactivated with several potential triggers like stress. If reactivated, retrograde transmission of the virus from the peripheral site to CNS along nerve axon can result in CNS infection which makes HSV the most common cause of viral encephalitis. In such cases, prompt laboratory diagnosis of HSV infection is essential for patient management and possible initiation of antiviral therapy and reducing the mortality rate. Quantitative PCR assays can estimate the extent of viral replication in CNS and would be helpful as a prognostic marker and in monitoring antiviral effects.
HSV 1/2 RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of HSV-1 and HSV-2.

Categories: Infectious Diseases, PCR Kits

Advantages of HSV RQ Kit

Ready to Use

The HSV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel and Yellow/VIC channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

HSV standards & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 0.5 copy/µl for the cloned target

Linear Range: 1 million to 2 copies/µl for the cloned target

Reaction type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains HSV Mix, Internal Control, 4 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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