Detection of Human Papilloma Virus (HPV)

Human Papilloma Virus (HPV) is a non-enveloped double stranded DNA virus from papillomaviridae family. There are about 150 different types of HPV. HPV infection is considered the main cause of cervical cancer and has been linked to more than 99% of all cases. According to data linking HPV infection to cervical cancer, HPV types have been divided into three groups of High-Risk, Low-Risk and No-Risk. Currently 14 types of 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 are considered as High-Risk and 6 types considered as Low-Risk including 6, 11, 42, 43, 44 and 53.
HPV HR RQ4 kit provides a ready-to-use Real-Time PCR assay for detection of HPV DNA including 14 High Risk Types of 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 and 2 Low Risk Types of 6 and 11.

Category: Infectious Diseases

Advantages of HPV HR RQ4 Kit

Ready to Use

The HPV HR RQ4 Mixes contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM, Yellow/VIC, Orange/ROX, Red/Cy5 channels.

Internal Control

The PCR mix contains primers and probe for a housekeeping gene as Internal Control that is detected in Orange/ROX channel. Internal
Control prevents false negative results due to extraction failure, reaction inhibition or setup errors.

Quality Control

HPV HR RQ4 mixes are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 50 copies/µl for the cloned target

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Multiplex (FAM, VIC, ROX, Cy5)

Kit Contents

Kit contains 4 HPV HR Mixes, Positive control, Negative control, PCR grade water, Manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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