Detection of HLA-B27

Human Leukocyte Antigen (HLA-B27) is a class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) on chromosome 6. HLA-B27 has been associated with ankylosing spondylitis and other inflammatory diseases such as reactive arthritis, uveitis, and psoriasis. Studies show 90% of patients with ankylosing spondylitis have HLA-B27 compared with 5%-10% of healthy controls. As a result, tests for HLA-B27 are valuable in the diagnosis and control of these diseases.
HLA-B27 RQ kit provides a ready-to-use Real-Time PCR assay for detection of HLA-B27.

Category: Genetic Factors

Advantages of HLA-B27 RQ Kit

Ready to Use

The HLA-B27 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be simply viewed in Green/FAM and Yellow/VIC channels.

Internal Control

To examine DNA extraction quality as well as the presence of PCR inhibitors and to prevent false negative results, primers and probe for an Internal Control (a housekeeping gene) is included in the PCR Master Mix and any false negative result due to above errors is prevented.

Quality Control

Mix & controls of HLA-B27 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument (RotorGene and StepOne).

Kit Specifications

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix, positive control, negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

Documents: