Detection and Quantitation of HIV-1

Human Immunodeficiency Virus (HIV) is a retrovirus with 2 strands of RNA as genome, each about 9.7 kb. Infection with this virus, affects the immune system and over the time leads to Acquired Immunodeficiency Syndrome (AIDS). There are two types of HIV, HIV-1 and HIV-2. Most of worldwide cases are due to HIV-1, while HIV-2 is mostly limited to West Africa.
HIV RQ kit provides a ready-to-use One-Step Real-Time RT-PCR assay for detection and quantitation of HIV-1 RNA (Group M subtypes A-H; and Group N).

Category: Infectious Diseases

Advantages of HIV RQ Kit

Ready to Use

The HIV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample RNA. Results can be viewed in Green/FAM channel and Yellow/VIC channel.

Internal Control

The PCR mix contains also specific DNA that is detected by a second probe in Yellow/VIC channel. Internal Control prevents false negative results by PCR inhibition or setup errors.

Quality Control

HIV standards & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 10 IU/µl for the cloned target

Linear Range: 50 million to 50 IU/µl for the cloned target

Reaction Type: Quantitative Real-Time RT-PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains HIV Mix, Internal control, 4 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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