Detection and Quantitation of HHV-6A and HHV-6B

Human Herpesvirus 6 (HHV-6) is a double-stranded DNA virus and a member of Herpesviridae family. It comprises two distinct herpesvirus species of HHV-6A and HHV-6B. Primary infection occurs mostly in early childhood followed by a life-long latent infection. While both viruses may get reactivated later on, HHV-6B is more prone to it especially in post-transplant patients. Both viruses have also been related to nervous system complications including meningitis and encephalitis.
HHV6 RQ kit provides a ready-to-use Real-Time PCR assay for detection, differentiation and quantitation of HHV-6A and HHV-6B.

Category: Infectious Diseases

Advantages of HHV6 RQ Kit

 Ready to Use

The HHV6 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM, Yellow/VIC and Orange/ROX channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

Reagents of HHV6 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 10 copies/µl for the cloned target

Linear Range: 100 million to 10 copies/µl for the cloned target

Reaction type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Triplex (FAM, VIC, ROX)

Kit Contents

Kit contains PCR Mix, Internal Control, 4 quantitation standards, water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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