Detection of H63D and C282Y mutations

Hereditary Hemochromatosis is a disorder resulted from iron overload in tissues. Some mutations on HFE gene, mostly H63D, C282Y and S65C can lead to this disease. Clinical features vary and may involve liver, heart, endocrine glands and skin.
Hemochromatosis RQ kit provides a ready-to-use Real-Time PCR assay for detection of H63D and C282Y mutations.

Category: Genetic Factors

Advantages of Hemochromatosis RQ Kit

Ready to Use

The Hemochromatosis Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be simply viewed in Green/FAM for H63D mutation and Yellow/VIC channels for C282Y mutation.

Internal Control

Due to dual function of the PCR Mix, the main reaction ensures quality of patient sample and DNA extraction and prevents false negative results by PCR inhibition or setup errors.

Quality Control

Mixes & controls of Hemochromatosis RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Differentiation: WW, WM and MM Genotypes

Reaction Type:  Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains 282/63 M Mix and 282/63 W Mix, MM Positive control, WM Positive control, WW Negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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