Detection and Quantitation of Hepatitis C Virus

Hepatitis C Virus (HCV) is an RNA virus and an important human pathogen. It is estimated that about 60 million individuals are infected worldwide. It can cause acute and chronic hepatitis followed by liver cirrhosis and hepatocellular carcinoma.
Currently infection can be diagnosed by detection of HCV RNA in patient sample by RT-PCR, weeks to months before antibody response. In addition, monitoring viral load by the same method has become a clinical gold standard for evaluation of therapy success as well as disease progress.
HCV RQ kit provides a ready to use One-Step Real-Time RT-PCR assay
for detection and quantitation of HCV RNA.

Category: Infectious Diseases

Advantages of HCV RQ Kit

Ready to Use

The HCV Mix contains all necessary reagents for Real-Time One-Step RT-PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample RNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative
results.

Quality Control
HCV Kits are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 1.5 IU/µl for the cloned target

Linear Range: 2.5 million to 5 IU/µl for the cloned target

Reaction Type: One-Step Quantitative Real-Time RT-PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains HCV Mix, Internal Control, 4 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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