Detection of Influenza A, Influenza B and Respiratory Syncytial Viruses

Human influenza A and B are enveloped RNA viruses of the Orthomyxoviridae family. While both of these viruses cause seasonal flu epidemics, Influenza A virus is responsible for majority of them and may also cause pandemics.
Respiratory Syncytial Virus (RSV) is also an enveloped RNA virus and belongs to Paramyxoviridae family. RSV mostly causes lower respiratory tract infections with cold like symptoms in healthy individuals, but it may also cause severe respiratory infections in some.
Flu-RSV RQ kit provides a ready-to-use One-Step Multiplex Real-Time RT-PCR assay for detection of Influenza A virus, Influenza B virus and RSV.

Category: Infectious Diseases

Description

Advantages of Flu-RSV RQ Kit

Ready to Use

The Flu-RSV Mix contains all necessary reagents for One-Step Real-Time RT-PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample RNA. Results are viewed in Green/FAM, Yellow/VIC, Orange/ROX and Red/Cy5 channels.

Internal Control

Kit also contains reagents for detection of RNase P as Internal Control which is detected in Orange/ROX channel. Internal Control prevents false negative results due to extraction failure, reaction inhibition or setup errors.

Quality Control

Control & mixes of Flu-RSV RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument, also reducing setup errors.

Kit specifications

Analytical sensitivity: 50 copies/µl for Influenza A virus, 250 copies/µl for Influenza B virus and 150 copies/µl for RSV

Reaction type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Multiplex (FAM, VIC, ROX, Cy5) 

Kit Contents

Kit contains PCR Mix and positive control and PCR grade water, manual, quick guide and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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