Detection of Factor XIII (Val34Leu) Polymorphism

Factor XIII, first called fibrin stabilizing factor, was discovered in 1948. It is involved in the last stage of coagulation and participates in clot preservation. It also participates in wound repair and healing, maintaining pregnancy and acts as proangiogenesis. Among the few polymorphisms known for this factor, the Val34Leu has been studied more extensively as it increases the activity of factor XIII and leads to change in fibrin clots. Studies have shown that this polymorphism may reduce the risk of myocardial infarction, brain infarction and venous thromboembolism (VTE). However, it also increases the risk of intracerebral hemorrhage and pulmonary embolism.
Factor XIII RQ kit provides a ready-to-use Real-Time PCR assay for detection of Factor XIII (Val34Leu) Polymorphism.

Category: Genetic Factors

Advantages of F13 RQ Kit

Ready to Use

The F13 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be simply viewed in
scatter graph analysis or other methods.

Internal Control

Due to dual function of the PCR Mix, the main reaction ensures quality of patient sample and DNA extraction and prevents false negative results by PCR inhibition or setup errors.

Quality Control

Mix & controls of Factor 13 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Differentiation: WW, WM and MM Genotypes

Reaction Type: Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains F13 Mix, MM Positive control, WM Positive control, WW Negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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