Detection of Factor V Leiden (G1691A)

Factor V Leiden is an inherited disorder of blood clotting and refers to specific G1691A substitution in Factor V gene (R506Q). It was first reported in 1994.
3 to 8 percent of Caucasian population carries one copy of this mutation (heterozygous) and about 1 in 5000 carry two copies of it (homozygous). Heterozygosity for factor V Leiden is associated with 4-8 fold increased risk of venous thromboembolism (VTE) including deep venous thrombosis (DVT) and pulmonary embolism. Factor V Leiden is also associated with a 2-3 fold increase in relative risk of pregnancy loss, and possibly other pregnancy complications such as preeclampsia, fetal growth retardation and placental abruption.
The diagnosis of factor V Leiden requires the APC resistance assay as a coagulation screening test or sequence analysis of factor V gene.
Factor V RQ kit provides a ready-to-use Real-Time PCR assay for detection of Factor V Leiden (G1691A).

Category: Genetic Factors

Advantages of F5 RQ Kit

Ready to Use

The F5 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent preparation or mixing is required.
Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be simply viewed in
scatter graph analysis or other methods.

Internal Control

Due to dual function of the PCR Mix, the main reaction ensures quality of patient sample and DNA extraction and prevents false negative results by PCR inhibition or setup errors.

Quality Control

Mix & controls of Factor V RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Differentiation: WW, WM and MM Genotypes

Reaction Type: Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains F5 Mix, MM Positive control, WM Positive control, WW Negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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