Detection of Toxoplasma gondii

Toxoplasma gondii is a parasitic protozoan that causes toxoplasmosis in warm-blooded animals. Cats are the definitive host for toxoplasma gondii but several intermediate hosts also exist. It is usually transmitted from infected meat or cat feces. Toxoplasmosis is asymptomatic in most humans but it causes various symptoms in immunocompromised patients, pregnant women and congenitally infected children with toxoplasma.

Toxoplasma Kit provides a ready-to-use Duplex Real-Time PCR assay for detection of Toxoplasma DNA. 

Category: Infectious Diseases

Advantages of Toxo RQ Kit

Ready to Use

The Toxo Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure, PCR inhibition or setup errors, preventing false negative results. Internal Control is detected in Yellow/VIC channel.

Quality Control

Control & mix of Toxo RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument, also reducing setup errors.

Kit specifications

Analytical sensitivity: 4 copies/µl for the cloned target.

Reaction type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC) 

Kit Contents

Kit contains PCR Mix, Internal Control, Positive control and PCR grade water, manual, quick guide and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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