Detection and Quantitation of Cytomegalovirus (CMV)

Human Herpesvirus 5 (HHV-5) known as Cytomegalovirus (CMV) is a double-stranded DNA virus and a member of Herpesviridae family. It is a widespread pathogen with high seropositivity (about 80%) in adult population. Primary infection is usually subclinical and mostly happens in early childhood. It is followed by the latent infection and CMV persists in the infected person through life. CMV can be reactivated in immunocompromised hosts, for example after stem cell, bone marrow or solid organ transplantation or in the course of HIV infection.
CMV RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of cytomegalovirus DNA.

Category: Infectious Diseases

Advantages of CMV RQ Kit

Ready to Use

The CMV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Internal Control

This Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control
CMV standards & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 1 copy/µl for the cloned target

Linear Range: 1 million to 1 copy/µl for the cloned target

Reaction type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains CMV Mix, Internal Control, 4 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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