Detection of BRAF Mutations

BRAF oncogene is among the most frequently mutated kinases in human cancer. Mutations in codon V600 have been reported in different types of cancers including 40%-50% of melanomas, 10%-70% of thyroid carcinomas, 10% of colorectal cancers and 3%-5% of Non-Small Cell Lung Cancers (NSCLC). Most of the BRAF mutations are located in codon 600 and constitute V600E, V600Ec, V600D, V600K, V600R.
BRAF RQ kit provides a ready-to-use Real-Time PCR assay for detection of these 5 mutations.

Category: Oncology biomarkers

Advantages of BRAF RQ Kit

 Ready to Use

The BRAF Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Quality Control

BRAF RQ Kit is manufactured in batches, and each series is distributed only after careful Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument.

Kit Specifications

Analytical Sensitivity: 0.5%-2%, Depending on the mutation type

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex/ Duplex (FAM, VIC)

Kit Contents

Kit contains two Control Mixes and five PCR Mixes including V600E, V600Ec, V600D, V600K and V600R for BRAF mutations, positive control, negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24 and 48 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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