Detection and Quantitation of BK Virus

BK Virus is a member of polyomavirus genus with a double stranded DNA genome. The virus is widespread with a seroprevalence of up to 90% in adults. Infection usually happens during the childhood and is either asymptomatic or with mild symptoms. Following initial infection, virus persists as a lifelong latent infection. BKV can cause significant consequences in renal or bone marrow transplant patients including polyomavirus associated nephropathy (PVAN) and graft loss. Quantitative monitoring of BKV load in plasma or urine of transplant recipients is essential and a load of 10,000 copies/ml in plasma or 10 million copies/ml in urine in 3 weeks or more is an early indication of PVAN.

BK RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of BK virus DNA.

Category: Infectious Diseases

Advantages of BK RQ Kit

 Ready to Use

The BK Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Internal Control

This Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

Standards & mixes of BK RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 2 copeis/µl for the cloned target

Linear Range: 10 million to 10 copies/µl for the cloned target.

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix, Internal Control and 4 quantitation standards and PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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