Detection and Quantitation of AML1-ETO Transcripts

AML1-ETO is the most common chromosomal aberration in de novo acute myeloid leukemia (AML) patients. This abnormality is resulted from t(8;21) (q22;q22) translocation. The translocation produces a fusion protein which inhibits myeloid transcriptional factors and as a result, cellular differentiation is blocked. This alteration occurs in approximately 7% of adult and 12% of pediatric with AML.
AML1-ETO RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of AML1-ETO transcripts as well as calculation of AML1-ETO percentage.

Category: Oncology biomarkers

Advantages of AML1-ETO RQ Kit

Ready to Use

The AML1-ETO and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for AML1-ETO and Yellow/VIC channel for ABL.

Control Gene

Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis, preventing related false negative results.

Quality Control

Standards & mixes of AML1-ETO RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 2 copies/µl or 0.02% for AML1-ETO, in context of 10,000 copies/μl of ABL transcripts

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix and 5 quantitation standards for AML1-ETO, PCR Mix and 4 quantitation standards for ABL and PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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