Detection and Quantitation of Adenovirus

Adenoviruses belong to the Adenoviridae family, comprising non-enveloped and dsDNA viruses. They cause mild infections in the upper or lower respiratory tract, digestive tract, and conjunctiva. Adenovirus is transmitted through the respiratory or fecal-oral route during close personal contact or contact with contaminated objects.

Adenovirus infection is more prevalent among young children while individuals with compromised immune systems (organ transplant recipients, people with human immunodeficiency virus, and people with congenital immunodeficiency) are at a higher risk of developing this infection. The incubation period ranges from 2 to 14 days. Early diagnosis is crucial for patient management.

Adenovirus RQ Kit provides a ready-to-use Real-Time PCR assay for the detection and quantitation of Adenovirus DNA.

Category: Infectious Diseases

Advantages of Adenovirus RQ Kit

Ready to Use

The Adeno Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in the Green/FAM and Yellow/VIC channels.

Internal Control

This Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

Mix and standards of Adenovirus RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 1 copy/µl for the cloned target

Linear Range: 10 million to 10 copies/µl for the cloned target.

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix, Internal Control and 4 quantitation standards and PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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