Detection and Quantitation of Hepatitis B Virus

Hepatitis B, a viral infection with Hepatitis B Virus (HBV) is a major global health issue. About 300 million people are infected worldwide.
HBV also contributes to an estimated 800,000 deaths every year. In recent years, accessing the level of circulating virus in blood has been regarded as the most reliable tool for disease monitoring and patient management including clinical staging and pretreatment evaluation, monitoring and evaluation of antiviral therapy success and detection of emerging new resistant viruses.
HBV RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of HBV DNA.

Category: Infectious Diseases

Advantages of HBV RQ Kit

Ready to Use

The HBV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control
HBV standards & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 0.15 IU/µl for the cloned target

Linear Range: 10 million IU/μl to 1 IU/μl

Reaction type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains HBV Mix, Internal Control, 5 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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