Detection of SARS-CoV-2, Human Influenza Virus A/B, Respiratory Syncytial Virus (RSV) and Human Parainfluenza Viruses 1-4 (HPIV1-4) RNA.

Coronaviruses are enveloped viruses characterized with single stranded RNA genome, which cause respiratory illnesses. The emergence of SARS (Sever Acute Respiratory Syndrome by SARS CoV), MERS (Middle East Respiratory Syndrome by MERS CoV), and recently COVID-19 (by SARS CoV-2), has highlighted their significant impact on global health.

Human influenza A and B are enveloped RNA viruses of the Orthomyxoviridae family. While both viruses cause seasonal flu epidemics, Influenza A virus is responsible for the majority of them and may also cause pandemics.

Respiratory Syncytial Virus (RSV) is also an enveloped RNA virus and belongs to Paramyxoviridae family. RSV mostly causes lower respiratory tract infections with cold like symptoms in healthy individuals, but it may also cause severe respiratory infections in some.

Human parainfluenza viruses are single-stranded RNA viruses classified under the paramyxoviridae family. These viruses are divided into four distinct types HPIV-1, HPIV-2, HPIV-3 and HPIV-4, each associated with different clinical symptoms and varying degrees of severity. Signs and symptoms are similar to common cold, but it can also cause more serious infection especially in children.

Resp II RQ kit provides a ready-to-use One-Step Multiplex Real-Time RT-PCR assay for detecting of SARS-CoV-2, Human Influenza Virus A/B, Respiratory Syncytial Virus (RSV) and Human Parainfluenza Viruses 1-4 (HPIV1-4) RNA.

Category: Infectious Diseases

Advantages of Resp II RQ Kit

Ready to Use

The Resp II Mix contains all necessary reagents for RT-PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample RNA. Results are viewed in Green/FAM, Yellow/VIC, Orange/ROX and Red/Cy5 channels.

Internal Control 

Kit also contains reagents for detection of RNase P as an Internal Control which is detected in Orange/ROX channel. Internal Control prevents false negative results due to the extraction failure, reaction inhibition or setup errors.

Quality Control

Controls & mixes of Resp II RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 13 copies/μl for SARS-CoV-2, 10 copies/μl for Influenza A and B, 9 copies/μl for RSV, 8 copies/μl for HPIV-1, 8.4 copies/μl for HPIV-2. 13.6 copies/μl for HPIV-3 and 9.2 copies/μl for HPIV-4.

Reaction type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Multiplex (FAM, VIC, ROX, Cy5)

Kit Contents
Kit contains Resp A Mix, Resp B Mix and Resp A positive control, Resp B positive control and PCR grade water, manual and a CD with template file.

Packaging
Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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