Detection of Leishmania

Leishmania is a parasitic protozoan that causes Leishmaniasis in vertebrates. Over 30 species of Leishmania have been known but some of them are more important as they can infect humans, such as; L. major, L. tropica, L. aethiopica, L. donovani and L. infantum. Phlebotomine sand flies are the intermediate host for Leishmania. Leishmaniasis is most prevalent in tropical and subtropical regions. Based on clinical symptoms, the three main phenotypic categories of disease are Cutaneous Leishmaniasis (CL), Visceral Leishmaniasis (VL), and Mucocutaneous Leishmaniasis (MCL). The infection could be asymptomatic or present with various clinical manifestations. Cutaneous Leishmaniasis is the most prevalent type of infection with Leishmaniasis. Since the symptoms of Visceral Leishmaniasis are similar to some common diseases such as tuberculosis, typhoid, and malaria, rapid and specific diagnosis of this parasite is crucial for effective treatment.

Leishmania Kit provides a ready-to-use Duplex Real-Time PCR assay for detection of Leishmania DNA. 

Category: Infectious Diseases

Advantages of Leishmania RQ Kit

Ready to Use

The Leishmania Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure, PCR inhibition or setup errors, preventing false negative results. Internal Control is detected in Yellow/VIC channel.

Quality Control

Control & mix of Leishmania RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument, also reducing setup errors.

Kit specifications

Analytical sensitivity: 4 copies/µl for the cloned target.

Reaction type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC) 

Kit Contents

Kit contains PCR Mix, Internal Control, Positive control and PCR grade water, manual, quick guide and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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