Detection of EGFR Mutations

Epidermal Growth Factor Receptor (EGFR), is a tyrosine kinase receptor and is considered as an oncogene. EGFR is involved in regulation of cellular proliferation, differentiation and survival. Mutations in EGFR exons 18, 19, 20 and 21 are associated with the development of different human cancers specially Non-Small Cell Lung Cancer (NSCLC) and glioblastoma. Since, the choice of anti-EGFR therapies are highly dependent on the EGFR mutations, it is essential that patients are tested for these mutations.

This kit provides ready to use reagents for detection of 34 mutations. Detected mutations include three point mutations in exon 18 (G719A, G719S and G719C without differentiating them), 24 deletions in exon 19 (without differentiation between them), three insertion (without differentiation between them) and two point mutations in exon 20 (S768I, T790M) and two-point mutations in exon 21 (L858R, L861Q). 

Category: Oncology biomarkers

Advantages of EGFR RQ Kit

Ready to Use

The EGFR Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Quality Control

EGFR RQ Kit is manufactured in batches, and each series is distributed only after careful Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument.

Kit Specifications

Analytical Sensitivity: 1%-8%, depending on the type of mutation type.

Reaction Type: Qualitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex/Duplex (FAM, VIC)

 Kit Contents

Kit contains two control Mixes and seven PCR Mixes including 19Del, G719X, 20Ins, S768I, T790M, L858R, L861Q for EGFR mutations, positive control, negative control, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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