Detection and Quantitation of BCR-ABL p190 Transcripts

(Philadelphia Chromosome)

Philadelphia chromosome is an abnormality resulted from 9;22 translocation. Consequently, ABL proto-oncogene on chromosome 9 is fused with BCR gene on chromosome 22. This fusion produces BCR-ABL protein, mostly 210kDa (b2a2 or b3a2) or 190kDa (e1a2), with constitutively active tyrosine kinase activity promoting cell proliferation and inhibition of apoptosis. The fusion gene transcript is detectable in about 95% of CML patients and some cases of ALL. Also, serial monitoring of patients for identifying and measuring BCR-ABL transcripts provides more precise assessment of response to specific therapies and prediction of those in higher risk of disease progression.
BCR-ABL (p190) RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of BCR-ABL transcripts (p190, e1a2 break point only) as well as calculation of BCR-ABL%.

Category: Oncogenetics

Advantages of mbcr p190 RQ Kit

Ready to Use

The mbcr and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for BCR-ABL and Yellow/VIC channel for ABL.

Control Gene

Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis, preventing related false negative results.

Quality Control

Standards & mixes of mbcr 190 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 10 copies/µl or 0.02%, in context of 5,000 copies/μl of ABL transcripts

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Singleplex (FAM, VIC)

Kit Contents

Kit contains mbcr 190 Mix and 5 quantitation standards for ABL Mix and 4 quantitation standards for ABL, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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