Detection and Quantitation of Epstein-Barr Virus (EBV)

Human Herpesvirus 4 (HHV-4) known as Epstein-Barr Virus (EBV) is a double-stranded DNA virus and a member of Herpesviridae family. Infection occurs mostly in early childhood and without clinical symptoms. The primary infection is followed by the lifelong latent infection and about 90% of adult population are infected with this virus. EBV has also been linked to a variety of cancers including lymphoma and nasopharyngeal carcinoma. It also can be reactivated in immunocompromised hosts, after transplantation or following therapy in cancer patients leading to lymphoproliferative disorders. Therefore, detection of EBV and viral load monitoring is important in these patients.
EBV RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of Epstein Barr virus.

Category: Infectious Diseases

Advantages of EBV RQ Kit

Ready to Use

The EBV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Internal Control

This kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

Standards & mix of EBV RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 0.5 copy/µl for the cloned target

Linear Range: 1 million to 1 copy/µl for the cloned target

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix and 4 quantitation standards and internal control for EBV Mix and PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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